Creating a Sample List (Sequence)

There are two basic ways how to create a sample list. (For more information about the sample list, refer to Samples and Sequences  The Sample List (Sequence)):

1. Manually edit an existing sequence and save it under a different name.

2. Automatically create a sequence using the Sequence Wizard. (For information about the wizard, refer to Samples and Sequences  The Sequence Wizard).

Manually creating a sample list

• In the Browser (see Data Management  The Browser), select the sequence whose QNT and PGM Files you want to use for the new sequence, probably after having edited them. Select Save as on the File menu to save the sequence under a different name. Edit the new sequence as necessary.

• In the lower right Browser section, enter the names and the properties of the standard samples and the unknown samples to be analyzed:

• Click a cell in the table and enter the new value or the name via the keyboard. To open a dialog box for assistance, press the F8 key.

• In the Name column, enter a name for each sample to be analyzed.

• In the Type column, select the sample type from the drop-down list (Unknown, Blank, Validate, Standard, Spiked, or Unspiked).

• In the Pos. (sample position) and Inj. Vol. (injection volume) columns, type the autosampler position from which to inject and the substance volume.

• In the Program and Method columns, determine the Chromatographic Methods to be used for the analysis.

• In the Status (sample status) column, define how often a sample shall be processed. Select Single to process the sample only once. Select Multiple to process the sample several times.

• To analyze two unknown samples (Sample 1 and 2) on the basis of a 2-point calibration, the input can be as follows:

• It is not imperative that you fill the other columns, e.g., Weight (Sample Weight Factor), Dil. Factor (dilution factor) and Inj. Date/Time. Either they contain special parameters or the system fills them automatically after the analysis.

• If you are not sure which values or names to enter, select the cell and press the F8 key. An edit dialog box appears listing the allowed values and/or options. Enter a value or select an option. Clicking OK automatically updates the cell.

• Press the F1 key to display more information about the individual columns.

• Press the F9 key to fill all subsequent cells of a column with the same input.

• You can have Chromeleon generate a unique ID for each sample (see  Using Globally Unique Sample Identifiers).

• To enter special values, create User-defined Columns (see  Creating User-defined Columns).

• To take an overview of the most important results in the sample list after the samples have been analyzed, add Sequence Report Columns (see  Creating a Sequence Report Column).

• To save the sequence under a new name, select Save As on the File menu. To save the sequence under the existing name, select Save.

• Check the sequence properties by pressing <ALT> + <ENTER>. Or else, select the sequence, and then select Properties on the context menu.

Automatically creating a sample list

• Use one of the following methods to open the Sequence Wizard:

 In the Browser, select New on the File menu. Select Sequence (using Wizard) from the list and click OK.

 In a panel tabset, click the Sequence Control tab. Under Create Application, click Create Sequence.

• Follow the instructions to create a basic sequence structure.

• Press the F1 key for more help.

For more information about the appearance of the sample list, refer to  Displaying Sequence Columns.

For information about the wizard, refer to Samples and Sequences  The Sequence Wizard.